text 2015 Jurnal Veteriner , Vol. 15(2)p.159-166 Enzyme-linked immunosorbent assay (ELISA) is a serological test method most widely used for thediagnosis of paratuberculosis, because it has a better sensitivity compared to other serological test.Protoplasmic antigen (PPA) or cellular extract is still the main choice for the diagnosis of paratuberkulosisdevelopment. The aim of research was to use the PPA Mycobacterium avium subspeciesparatuberculosis(MAP) field isolates for the development of paratuberculosis ELISA (ELISA PPA-L). As many as 322cattle sera (300 negative and 22 positive) were tested using this method and compared with IDEXXcommercial kit. The sensitivity and specificity of ELISA PPA-L test results were 68.18% and 97.0%,whereas for the IDEXX kit were 63.64% and 97.33%respectively. ELISA PPA-L had higher sensitivity andlower specificity compared to the IDEXX commercial kit. ELISA test using protoplasmic antigen of MAPfield isolates has good ability for paratuberculosis serological test and can be used for screening test of thedisease in Indonesia. Adji, Rahmat Setya;Wawan, I WayanTeguh ; Lukman, Denny Widaya :Setiyaningsih, Surachmi ELISA, PPA, paratuberculosis, Mycobacterium avium subspecies paratuberculosis ARTVET2316 ARTVET2316 210830 20210830011812 INLIS000000000017736 Converted from MARCXML to MODS version 3.5 using MARC21slim2MODS3-5.xsl (Revision 1.106 2014/12/19)