text 2015 Jurnal Veteriner Vol.16 (1), p.1-7 Bovine viral diarrhea virus (BVDV) is an important viral disease, which a ubiquitous pathogen of cattle with worldwide economic importance and due to its misdiagnose with other viruses. The goal of the current study was to identify and characterize of BVDV by reverse transcriptase polymerase chain reaction (RT-PCR) and followed by sequence genome analyses. Blood, feces, and semen samples were collected from 588 selected cattle from animals suffering from diarrhea and respiratory manifestation. RT- PCR results showed that the 69 (11.74%) samples were positive to BVDV. Further molecular characterization was conducted only with 17 PCR positive samples. The results indicated the 17 Indonesian BVD virus isolates were belonging to the genotype-1 of BVDV (BVDV-1) based on sequence analysis and a phylogenetic relationship between Indonesian BVDV isolates and BVDV in the world. This finding is the first report of BVD-1 circulated in Indonesian cattle Saepulloh, Muharam; Sendow, Indrawati (Indonesian Research Center for Veterinary Science) BVDV, RT-PCR, genotype-1 ARTVET2068 ARTVET2068 210902 20210902015426 INLIS000000000017722 Converted from MARCXML to MODS version 3.5 using MARC21slim2MODS3-5.xsl (Revision 1.106 2014/12/19)