Sendow, I text 1994 Penyakit Hewan Vol. 26 (48), p. 21-25. 1994 Bluetongue virus serotype 21 was isolated from a pool of Aedes app. and Anopheles spp. mosquitoes collected at a low altitude site in West Java in a light trap used for collecting Culicoides spp. A suspension prepared from the mixed pool was inoculated firstly into embryonated chicken eggs before passaging into Aedes alhophius (C6/36) and baby hamster kidney (I311K -21) cells. Viral isolates were screened by immunodot blotting using a monoclonal antibody specific for bluetongue group viruses. Those reacting were submitted to the international reference laboratory at Onderstepoort for confirmation and scrotyping. Sendow, I BTV type 21, mosquitoes, isolation ARTVET2055 ARTVET2055 241112 20241112103054 INLIS000000000017709 Converted from MARCXML to MODS version 3.5 using MARC21slim2MODS3-5.xsl (Revision 1.106 2014/12/19)