na INLIS000000000017709 20241112103054 241112 | | | 0010-0721002241 ARTVET2055 ARTVET2055 Sendow, I Isolation of Bluetongue Virus Serotype 21 from Mosquitoes in West Java, Indonesia / Sendow, I Penyakit Hewan 1994 Vol. 26 (48), p. 21-25. 1994 Bluetongue virus serotype 21 was isolated from a pool of Aedes app. and Anopheles spp. mosquitoes collected at a low altitude site in West Java in a light trap used for collecting Culicoides spp. A suspension prepared from the mixed pool was inoculated firstly into embryonated chicken eggs before passaging into Aedes alhophius (C6/36) and baby hamster kidney (I311K -21) cells. Viral isolates were screened by immunodot blotting using a monoclonal antibody specific for bluetongue group viruses. Those reacting were submitted to the international reference laboratory at Onderstepoort for confirmation and scrotyping. BTV type 21, mosquitoes, isolation. Daniels, P. W. Soleha, E. Sukarsih ARTVET2055