Sendow, Indrawati text 1993 Penyakit Hewan, Vol. 25 (46 A), p. 68-71. 4 A competitive enzyme-Jinked immunosorbent assay (C-ELISA) using a group specific monoclonal antibody (MAb) against the bluetongue viruses (B'IV) was established to detect antibodies against B1V. Sera from sentinel cattle in West Java were tested. The results of the C-ELISA were compared with the classical method for detection of antibodies to the bluetongue group, the agar gel immunodiffusion test. Increased specificity was observed using the Mab in the B1V C-ELISA. Not all sera that reacted in the AGID test reacted in the C-ELISA, and this was interpreted as the elimination of cross-reactions to other orbiviruses, including epizootic haemorrhagic disease of deer viruses, that are a problem in the agar gel immunodiffusioa (AGID) test. Increased sensitivity with this test was also observed as seroconversions to B1V were detected earlier by C-EUSA than with theAGID. The CELISA not only has increased sensitivity and speci~city, but also cost less, and can be used for sera from all species, and should replace the AGID test for bluetongue serology Sendow, Indrawati Elisa, bluetongue virus ARTVET2050 ARTVET2050 241015 20241015072642 INLIS000000000017704 Converted from MARCXML to MODS version 3.5 using MARC21slim2MODS3-5.xsl (Revision 1.106 2014/12/19)