01637     2200205   4500001002100000005001500021035002000036245008900056250004600145260000900191300000600200084001500206082001500221008003900236100002200275650002800297700001900325520107200344990001501416INLIS00000000001770420241015072642  a0010-07210022361 aApplication of Elisa to Detect Bluetongue Virus Group Infection /cSendow, Indrawati  aPenyakit Hewan, Vol. 25 (46 A), p. 68-71.  c1993  a4  aARTVET2050  aARTVET2050241015                |          | |  0 aSendow, Indrawati 4aElisa, bluetongue virus  aDaniels, Peter  aA competitive enzyme-Jinked immunosorbent assay (C-ELISA) using a group specific monoclonal antibody (MAb) against the bluetongue 
viruses (B'IV) was established to detect antibodies against B1V. Sera from sentinel cattle in West Java were tested. The results of the C-ELISA were
compared with the classical method for detection of antibodies to the bluetongue group, the agar gel immunodiffusion test. Increased specificity was
observed using the Mab in the B1V C-ELISA. Not all sera that reacted in the AGID test reacted in the C-ELISA, and this was interpreted as the
elimination of cross-reactions to other orbiviruses, including epizootic haemorrhagic disease of deer viruses, that are a problem in the agar gel
immunodiffusioa (AGID) test. Increased sensitivity with this test was also observed as seroconversions to B1V were detected earlier by C-EUSA
than with theAGID. The CELISA not only has increased sensitivity and speci~city, but also cost less, and can be used for sera from all species, and 
should replace the AGID test for bluetongue serology  aARTVET2050