02035     2200217   4500001002100000005001500021035002000036245025400056700008200310700001800392700002100410250003800431260000900469300002600478084001500504082001500519008003900534650007000573520115900643990001501802INLIS00000000001770020210929081245  a0010-0721002232  aVaccination Trial In Sheep Against Chrysomya Bezziana Larvae Using The Recombinant Perithrophin Antigens Cb15, Cb42 And Cb48. /cSukarsih; Partoutomo, Sutijono[Research Institute for Veterinary Science]Wijffels, Gene; Vuocolo, Tony; Willadse, Peter.  aPartoutomo, Sutijono[Research Institute for Veterinary Science]Wijffels, Gene  aVuocolo, Tony  aWilladse, Peter.  aJurnal Ilmu Ternak dan Veteriner.  c2000  aVol.5 (3): p.192-196.  aARTVET2046  aARTVET2046210929                |          | |   4aChrysomya bezziana, recombinant antigen, peritrophin, vaccination  aRecombinant forms of a number of peritrophic membrane proteins from the screwworm fly Chrysomya bezziana have been
assessed in vitro and in vivo for their efficacy as antigens in vaccination against the tissue-invasive, larval form of the parasite.
The proteins included Cb15 and Cb42 expressed in Escherichia coli and Cb48 expressed in both Escherichia coli and Pichia
pastoris. In all cases, the in vitro assays of larval growth on serum from vaccinated sheep failed to show inhibition of larval
weight gain or any detrimental effect on larval survival relative to controls. Chrysomya bezziana Cb48 has a significant degree
of sequence identity with the antigen PM48 from Lucilia cuprina. Feeding Lucilia cuprina larvae on antisera to Cb48 induced a
small but statistically significant reduction in weight gain, as does feeding on antisera to PM48. In vivo, larvae feeding on sheep
vaccinated with Escherichia coli-expressed Cbl 5 and Cb42 and Pichia pastoris-expressed Cb48 showed marginally greater
weight gain and survival which was equal to or greater than that on non-vaccinated sheep. The significance of these observations
is discussed.  aARTVET2046