01843     2200205   4500001002100000005001500021035002000036245018700056700001800243700008500261250003800346260000900384300003200393084001500425082001500440008003900455650008800494520104000582990001501622INLIS00000000001769720211004094123  a0010-0721002229  aBacterial expression of larval peritrophins of Chryosomya bezziana. /cWijffels, Gene; Vuocolo, Tony; Muharsini, Sri[Research Institute for Veterinary Science]Supriyanti, Florentina.  aVuocolo, Tony  aMuharsini, Sri[Research Institute for Veterinary Science]Supriyanti, Florentina.  aJurnal Ilmu Ternak dan Veteriner.  c2000  aVol.5 (3): p.170-176. 2000.  aARTVET2043  aARTVET2043211004                |          | |   4aChrysomya, peritrophic membrane, peritrophin, recombinant protein, hexaHis tag, GST  aThree candidate antigens, Chrysomya bezziana peritrophin-48,
Chrysomya bezziana
peritrophin-15 and Chrysomya bezziana
peritrophin-42, were prepared for recombinant protein production in
Escherichia coli
using a variety of expression vectors. Cb
peritrophin-48 was expressed as a recombinant protein possessing a carboxy-terminal hexaHis tag. Cb peritrophin-15 was
expressed as both a glutathione S-transferase fusion protein and as an amino-terminal hexaHis tagged protein. The glutathione Stransferase
Cb peritrophin-15 construct produced a heterogeneous group of fusion proteins. Cb peritrophin-42 was also
expressed as an amino-terminal hexaHis tagged protein. The two putative domains of Cb peritrophin-42 were also separately
expressed, again with amino-terminal hexaHis tags. Cultures of the hexaHis constructs Cb peritrophin-48, -15 and —42 were
demonstrated to be useful for the production and purification of these protein antigens and were scaled-up for vaccine trials and
protein characterization studies.  aARTVET2043