02550     2200217   4500001002100000005001500021035002000036245021500056700002700271700001700298700007700315250003900392260000900431300002700440084001500467082001500482008003900497650008800536520169300624990001502317INLIS00000000001769020211004105238  a0010-0721002222  aEffectivity of Lactobacillus plantarum BSL against Listeria monocytogenes in rats /cFirat Meiyasa; Betty Sri Laksmi Jenie; Lilis Nuraid; Wahyuwardani, Sutiastuti(Balai Penelitian Veteriner, Bogor (Indonesia)).  aBetty Sri Laksmi Jenie  aLilis Nuraid  aWahyuwardani, Sutiastuti(Balai Penelitian Veteriner, Bogor (Indonesia)).  aMalaysian Journal of Microbiology.  c2018  aVol 14 (3); p. 282-292  aARTVET2036  aARTVET2036211004                |          | |   4aLactic acid Bacteria, L. plantarum BSL, L. monocytogenes, probiotic, anti listerial  aAims: To evaluate the effectivity of Lactobacillus plantarum BSL isolated from Indonesian sauerkraut against Listeria
monocytogenes ATCC 7644 through in vitro and in vivo assay. 
Methodology and results: In vitro examination for antimicrobial activity against L. monocytogenes ATCC 7644 was
performed using seven isolates of lactic acid bacteria (LAB). Lactobacillus plantarum BSL demonstrated the highest
activity against L. monocytogenes and studied further in Sprague-Dawley (SD) rats. Treatment group of rats received
0.5 mL culture suspension (10
9
 CFU/mL) of L. plantarum BSL and control group received 0.5 mL of 0.85% w/v NaCl
daily during nine days of treatment. Both groups were infected at 3rd day with 0.5 mL of suspension of L.
monocytogenes (10
9
 CFU/mL). At the 2
nd
 (before infection), 5
th
, 7
th
, and 9
th
 day (after infection), the rats were sacrificed
and the faeces, caecum, and caecum content were examined for the population of LAB and L. monocytogenes.
Administration of L. plantarum BSL significantly increased the population of LAB by 1.2–1.4 log unit, while the number of
L. monocytogenes was reduced by 1.8–1.9 log unit compared to control group eithr in the faeces, caecum, or caecum
content. Administration of L. plantarum BSL could be able to reduce the liver and spleen damage of the experimental
rats, but did not show any changes in immunoglobulin A (IgA) response in comparison with control group. 
Conclusion, significance and impact of study: Lactobacillus plantarum BSL was promising as probiotic candidate
with health promotion to protect the gastrointestinal from infection by L. monocytogenes ATCC 7644.  aARTVET2036