02611     2200241   4500001002100000005001500021035002000036245018800056700002000244700001900264700001800283700002200301700001700323250013100340260000900471300001900480084001500499082001500514008003900529650004800568520173800616990001502354INLIS00000000001761120221216022806  a0010-0721002143  aSurra Cases in East Sumba Examined by Parasitological,Serological and Molecular Methods /cDewi, R. S.; Wardhana, A. H.; Sawitri, D. H.; Akbari, R. A.; Soejoedono, R. D.; Mulatsih, S.  aWardhana, A. H.  aSawitri, D. H.  aAkbari, R. A.  aSoejoedono, R. D.  aMulatsih, S.  aProceedings of International Seminar on Livestock Production and Veterinary Technology. Kualanamu, Medan. 16-20th October 2018  c2018  aP. 80-88. 2018  aARTVET2243  aARTVET2243221216                |          | |   4aSurra; East SUMBA; CATT; Trypanosoma evansi  aTrypanosoma evansi infection (Surra) is the most important disease Trypanosoma evansi infection (Surra) is the most important disease attacking livestock in Indonesia. In 2010-2012, outbreak of Surra occurred in Sumba Island where was previously reported as free region from Surra. It generated thousands livestock dead, mostly horses and buffaloes. The aim of the study was to use the parasitological (Giemsa Stained Blood Smears), serological (CATT T. evansi) and molecular techniques (PCR) for detection and confirmation of Surra cases in both horses and buffaloes occurred in Pahunga Lodu Distrit, East Sumba Regency. Blood samples of 20 horses and 20 buffaloes from traditional farm raised on extensive system were used in the study. Those three methods were applied. For molecular technique, ITS-1 primers were employed for 480 bp of DNA fragment from T. evansi. The results revealed that 4 samples (25.0%) of horses and 4 samples (33.3%) of buffaloes were positive microscopically and molecularly, while 10 samples (50%) of horses and 9 samples (75%) of buffaloes were found positive through CATT T. evansi test. It indicated that all positive horses and buffaloes might be in the active phase of Surra. The numbers of parasites found in the blood smears were relatively high, suggesting high parasitemia level. All livestock were treated with diminazene aceturate after investigation. It is concluded that Surra in East Sumba Regency remains a primary problem and PCR technique can be applied in the field to detect and confirm T. evansi, specially horses and buffaloes when the parasitemia is low. In addition, this technique is able to detect minute amounts of trypanosomal DNA in infected and apparently healthy livestock.  aARTVET2243