na INLIS000000000016945 20241111020029 241111 | | | 0010-0721001477 ARTVET1477 ARTVET1477 Adji, Rahmat Setya Pengembangan teknik diagnosa paratuberkulosis dengan Enzym Linked Immunosorbent Assay. / Adji, Rahmat Setya Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. (Balai Penelitian Veteriner, Bogor Indonesia), 2006 Bogor. 5-6 September 2006. p. 256 - 259. Diagnosis Technique Development Paratuberkulosis With Enzyme Linked Immunosorbent Assay. Paratuberculosis (Johne’s Disease) is a chronic granulomatous enteritis diseases of ruminants caused by Mycobacterium paratuberculosis. The disease spreads by faeces with clinical signs of progressive diarrhoae and weight losses. Diagnostic kit for paratuberculosis testing be needed for control and monitoring. The aim of research for develop of ELISA to diagnosis of paratuberculosis desease. The ELISA was developed have 0,29 of a cut – off value with both positive and negative control be tested by using commercial ELISA kit (IDEXX), showed good result. The result of ELISA 80 serum sample of dairy cattle from Kaliadem, Sleman-Daerah Istimewa Yogyakarta, showed negative paratuberculosis (mean of OD 0,162 ± 0,015). http://dx.doi.org/10.35726/jp.v16i1.44 ARTVET1477