02133     2200205   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113245030300128260000900431300003200440700002000472700015100492520124400643250002501887990001501912INLIS00000000001681920241111084209  a0010-0721001351ta241111                |          | |    aARTVET1351  aARTVET1351  aDetection of corpoantigens by sandwich ELISA in sheep experimentally infected with Fasciola gigantica. /cEstuningsih, Sarwitri Endah; Widjayanti, Sri; Adiwinata, Gatot( Balai Besar Penelitian Veteriner, Bogor Indonesia)Piedrafita, D.(School of Veterinary Science, Melbourne University, Australia).  c2004  aSupplement p.51-56. (2004).  aWidjayanti, Sri  aAdiwinata, Gatot( Balai Besar Penelitian Veteriner, Bogor Indonesia)Piedrafita, D.(School of Veterinary Science, Melbourne University, Australia).  aTwo monoclonal antibodies to Fasciola gigantica excretory/secretory (ES) antigens 
were used in a sandwich ELISA for the detection of Fasciola antigens in faeces of 9 sheep 
experimentally infected with 300 metacercariae of F gigantica. The detection of coproantigens 
was found in four of the seven sheep within 5 weeks of infection, and within 7 weeks of 
infection coproantigens were detected in all seven of the sheep. This technique was compared to 
an indirect ELISA for the detection of anti-Fasciola ES antigen specific antibodies in serum. The 
anti-F gigantica antibodies were detected within 3 weeks of infection in all of the infected 
sheep, suggesting a greater sensitivity to detect early infections. However, following 
anthelmintic treatment and removal of parasites, the anti-Fasciola antibody levels still 
remained high for at least 6 weeks when the study was terminated. In contrast, the levels of 
coproantigens were no longer detected in the faeces within 2 weeks of anthelmintic treatment. 
This study demonstrates that our sandwich ELISA for the detection of Fasciola coproantigens 
is able to detect immature fluke infections and more importantly, was able to detect patent 
infection of fasciolosis.  aTropical Biomedicine  aARTVET1351