02408     2200205   4500001002100000005001500021035002000036008004100056041000700097082000900104090001300113100001800126700002800144700001800172700002700190245010300217260004600320300002800366500180800394INLIS00000000001517420240212023944  a0010-0521015174240212                |          |        aen  aNONE  aMFN/23340 aLilis Nuraida0 aSutiastuti Wahyuwardani0 aFirat Meiyasa0 aBetty Sri Laksmi Jenie1 aEffectivity of Lactobacillus plantarum BSL against Listeria monocytogenes in rats /cLilis Nuraida  bMalaysian Society for Microbiology,c2018  aVol. 14(3), pp. 282-292  aAims: To evaluate the effectivity of Lactobacillus plantarum BSL isolated from Indonesian sauerkraut against Listeria monocytogenes ATCC 7644 through in vitro and in vivo assay. Methodology and results: In vitro examination for antimicrobial activity against L. monocytogenes ATCC 7644 was performed using seven isolates of lactic acid bacteria (LAB). Lactobacillus plantarum BSL demonstrated the highest activity against L. monocytogenes and studied further in Sprague-Dawley (SD) rats. Treatment group of rats received 0.5 mL culture suspension (109 CFU/mL) of L. plantarum BSL and control group received 0.5 mL of 0.85% w/v NaCl daily during nine days of treatment. Both groups were infected at 3rd day with 0.5 mL of suspension of L. monocytogenes (109CFU/mL). At the 2 nd(before infection), 5 th, 7th, and 9 th day (after infection), the rats were sacrificed and the faeces, caecum, and caecum content were examined for the population of LAB and L. monocytogenes. Administration of L. plantarum BSL significantly increased the population of LAB by 1.2–1.4 log unit, while the number of L. monocytogenes was reduced by 1.8–1.9 log unit compared to control group eithr in the faeces, caecum, or caecum content. Administration of L. plantarum BSL could be able to reduce the liver and spleen damage of the experimental rats, but did not show any changes in immunoglobulin A (IgA) response in comparison with control group. Conclusion, significance and impact of study: Lactobacillus plantarum BSL was promising as probiotic candidate with health promotion to protect the gastrointestinal from infection by L. monocytogenes ATCC 7644.