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Judul Molecular detection of extended-spectrum ?-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia
Pengarang Meutia Hayati
Agustin Indrawati
Ni Luh Putu Ika Mayasar
Istiyaningsih
Neneng Atikah
Penerbitan Surabaya : Veterinary World, 2019
Deskripsi Fisik 6 hlm. :ill.
ISSN 2231-0916
Subjek ?Klebsiella pneumoniae
BACTERIA
RESPIRATORY DISEASES
CHICKEN
ANTIBIOTIC
Abstrak Background and Aim: ?Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae may be transferred to other bacteria. One of the known resistance genes is extended-spectrum ?-lactamase (ESBL). This research aimed to study K. pneumoniae isolated from chicken farms in East Java, Indonesia, by observing the antibiotic resistance pattern and detect the presence of ESBL coding gene within the isolates. Materials and Methods: A total of 11 K. pneumoniae isolates were collected from 141 chicken cloacal swabs from two regencies in East Java. All isolates were identified using the polymerase chain reaction method. Antimicrobial susceptibility was determined by agar dilution method on identified isolates, which then processed for molecular characterization to detect ESBL coding gene within the K. pneumoniae isolates found. Results: The result of antibiotic se
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Lokasi Akses Online https://www.veterinaryworld.org/Vol.12/April-2019/14.pdf

 
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1Veterinary World, 12(4): 578-583, 20192026-03-091
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245 1 # $a Molecular detection of extended-spectrum ?-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia
260 # # $a Surabaya :$b Veterinary World,$c 2019
300 # # $a 6 hlm. : $b ill.
520 # # $a Background and Aim: ?Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae may be transferred to other bacteria. One of the known resistance genes is extended-spectrum ?-lactamase (ESBL). This research aimed to study K. pneumoniae isolated from chicken farms in East Java, Indonesia, by observing the antibiotic resistance pattern and detect the presence of ESBL coding gene within the isolates. Materials and Methods: A total of 11 K. pneumoniae isolates were collected from 141 chicken cloacal swabs from two regencies in East Java. All isolates were identified using the polymerase chain reaction method. Antimicrobial susceptibility was determined by agar dilution method on identified isolates, which then processed for molecular characterization to detect ESBL coding gene within the K. pneumoniae isolates found. Results: The result of antibiotic sensitivity test in 11 isolates showed highest antibiotic resistance level toward ampicillin, amoxicillin, and oxytetracycline (100%, 100%, and 90.9%) and still sensitive to gentamicin. Resistance against colistin, doxycycline, ciprofloxacin, and enrofloxacin is varied by 90.9%, 54.5%, 27.3%, and 18.2%, respectively. All isolates of K. pneumoniae were classified as multidrug resistance (MDR) bacteria. Resistance gene analysis revealed the isolates harbored as blaSHV (9.1%), blaTEM (100%), and blaCTX-M (90.9%). Conclusion: All the bacterial isolates were classified as MDR bacteria and harbored two of the transmissible ESBL genes. The presence of antibiotic resistance genes in bacteria has the potential to spread its resistance properties.
650 # 4 $a ?Klebsiella pneumoniae
650 # 4 $a ANTIBIOTIC
650 # 4 $a BACTERIA
650 # 4 $a CHICKEN
650 # 4 $a RESPIRATORY DISEASES
700 0 # $a Agustin Indrawati
700 0 # $a Istiyaningsih
700 0 # $a Neneng Atikah
700 0 # $a Ni Luh Putu Ika Mayasar
856 # # $a https://www.veterinaryworld.org/Vol.12/April-2019/14.pdf
990 # # $a 08/R/2026
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1 Molecular detection of extended-spectrum ?-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java Indonesia.pdf Molecular detection of extended-spectrum ?-lactamase-producing Klebsiella pneumoniae pdf Baca Online
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