| Judul | Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island |
| Pengarang | Ernes Andesfha Agustin Indrawat Ni Luh Putu Ika Mayasari Irma Rahayuningtyas Istiyaningsih Jusa |
| Penerbitan | JOURNAL OF ADVANCED VETERINARY AND ANIMAL RESEARCH, 2019 |
| Deskripsi Fisik | 10 hlm. :ill. |
| ISSN | 2311-7710 |
| Subjek | Salmonella BACTERIA Detection Chicken |
| Abstrak | Objective: The incidence of salmonellosis in humans and animals is still high due to the occurrence of virulence factors in Salmonella enterica which play a role in the process of infection in the host and the spread of disease and most of the S. enterica can infect humans and animals. The present study was aimed to identify Salmonella Enteritidis and detect virulence genes related to Salmonella pathogenicity islands (SPIs) and Salmonella plasmid virulence (Spv). Materials and Methods: A total of 27 S. Enteritidis archive isolates belonging to the National Veterinary Drug Assay Laboratory (NVDAL) were used in this study. The bacteria were collected in 2016 and 2017 from samples of the cloaca and fecal swabs from layer and broiler farms in five provinces of Java Island. Isolates were cultured in specific media, biochemical tests and Gram staining. Detection of S. Enteritidis and virulence genes was done by polymerase chain reaction (PCR) method. Results: Identification of serovar showed 100% (27/27) |
| Bentuk Karya | Tidak ada kode yang sesuai |
| Target Pembaca | Tidak ada kode yang sesuai |
| Lokasi Akses Online | http://bdvets.org/javar/ |
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| 001 | INLIS000000000001398 | ||
| 005 | 20260305102429 | ||
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| 022 | # | # | $a 2311-7710 |
| 035 | # | # | $a 0010-0326000005 |
| 082 | # | # | $a 636.09 |
| 084 | # | # | $a 636.09 ERN d |
| 100 | 0 | # | $a Ernes Andesfha |
| 245 | 1 | # | $a Detection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island |
| 260 | # | # | :$b JOURNAL OF ADVANCED VETERINARY AND ANIMAL RESEARCH,$c 2019 |
| 300 | # | # | $a 10 hlm. : $b ill. |
| 520 | # | # | $a Objective: The incidence of salmonellosis in humans and animals is still high due to the occurrence of virulence factors in Salmonella enterica which play a role in the process of infection in the host and the spread of disease and most of the S. enterica can infect humans and animals. The present study was aimed to identify Salmonella Enteritidis and detect virulence genes related to Salmonella pathogenicity islands (SPIs) and Salmonella plasmid virulence (Spv). Materials and Methods: A total of 27 S. Enteritidis archive isolates belonging to the National Veterinary Drug Assay Laboratory (NVDAL) were used in this study. The bacteria were collected in 2016 and 2017 from samples of the cloaca and fecal swabs from layer and broiler farms in five provinces of Java Island. Isolates were cultured in specific media, biochemical tests and Gram staining. Detection of S. Enteritidis and virulence genes was done by polymerase chain reaction (PCR) method. Results: Identification of serovar showed 100% (27/27) isolates were positive for the sdfI gene (304 bp). The result confirmed that all strains were S. Enteritidis. PCR based detection of virulence genes showed that 100% of isolates had virulence genes in SPI-1 to SPI-5, namely, invA, ssaQ, mgtC, spi4D, and pipA genes. All the isolates (27/27) were also positive to spvB gene-based PCR. Conclusion: All the isolates of S. Enteritidis in this study carry virulence genes related to SPI-1 to SPI-5 and plasmid virulence. The existence of virulent genes indicates that the S. Enteritidis strain examined in this study is highly virulent and poses a potential threat of worse disease outcome in humans and animals. |
| 650 | # | 4 | $a BACTERIA |
| 650 | # | 4 | $a Chicken |
| 650 | # | 4 | $a Detection |
| 650 | # | 4 | $a Salmonella |
| 700 | 0 | # | $a Agustin Indrawat |
| 700 | 0 | # | $a Irma Rahayuningtyas |
| 700 | 0 | # | $a Istiyaningsih Jusa |
| 700 | 0 | # | $a Ni Luh Putu Ika Mayasari |
| 856 | # | # | $a http://bdvets.org/javar/ |
| 990 | # | # | $a 05/R/2026 |
| No | Nama File | Nama File Format Flash | Format File | Action |
| 1 | Detection of Salmonella pathogenicity island and Salmonella plasmid virulance genes in Salmonella Eenteridis originated from layer and broiler farms in Java Island.pdf | Detection of Salmonella pathogenicity island | Baca Online |
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