Judul | Development of an Enzyme linked immunosorbent assay for detection of antibody against pasteurella multocida in chickens |
Pengarang | Siti Mariana ROBERT HIRST |
EDISI | vol.6 p.7-10 |
Penerbitan | Bogor BBPMSOH 1999 |
Deskripsi Fisik | 5 tables; 11 ref. Summary (En) |
ISBN | 0852-9612 |
Subjek | Elisa -- Spesific Pathogen Free State -- Vaccines -- CHICKENS -- ANTIBODIES -- MULTOCIDA -- ANTIGEN -- PASTEURELLA MULTOCIDA |
Catatan | In order to obtain a simple, cheap and sensitive method for detecting an antibody response in vaccinated and non-vaccinated chickens an Elisa technique was developed using heat stable antigen from Pasteurella multocida P-1662 as a coating antigen. The Elisa system in this study found to be sensitive however non-spesific reaction need to be avoided. Chickens with high antibody titer need to be diluted in 1/1000, while a dilution of 1/100 could be satisfactorily used for monitoring SPF chickens for laboratory purpose. |
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No Barcode | No. Panggil | Akses | Lokasi | Ketersediaan |
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Tag | Ind1 | Ind2 | Isi |
001 | INLIS000000000000087 | ||
005 | 20210323091256 | ||
008 | 210323||||||||| | ||| |||| || | | ||
020 | $a 0852-9612 | ||
035 | 0010-0321000087 | ||
041 | $a id | ||
100 | 0 | $a Siti Mariana | |
245 | 0 | 0 | $a Development of an Enzyme linked immunosorbent assay for detection of antibody against pasteurella multocida in chickens |
250 | $a vol.6 p.7-10 | ||
260 | $a Bogor $b BBPMSOH $c 1999 | ||
300 | $a 5 tables; 11 ref. Summary (En) | ||
500 | $a In order to obtain a simple, cheap and sensitive method for detecting an antibody response in vaccinated and non-vaccinated chickens an Elisa technique was developed using heat stable antigen from Pasteurella multocida P-1662 as a coating antigen. The Elisa system in this study found to be sensitive however non-spesific reaction need to be avoided. Chickens with high antibody titer need to be diluted in 1/1000, while a dilution of 1/100 could be satisfactorily used for monitoring SPF chickens for laboratory purpose. | ||
650 | 0 | $a Elisa -- Spesific Pathogen Free State -- Vaccines -- CHICKENS -- ANTIBODIES -- MULTOCIDA -- ANTIGEN -- PASTEURELLA MULTOCIDA | |
700 | 0 | $a ROBERT HIRST |
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