01179     2200181   4500001002100000005001500021008004100036035001900077041000700096100001900103700002800122245007300150250002100223260002500244300003400269500063600303650005800939INLIS00000000000012120210323091304210323|||||||||   |   |||   |||| ||   |   0010-0321000121  aen0 aUnang Patriana0 aAriestiana Permata Adji00aPenentuan Sulfadiazin dengan Kromatografi Cair Kinerja Tinggi (HPLC)  aVol. 2, p. 47-49  aBogorbBBPMSOHc1990  a3 tables; 4 ref. Summary (En)  aa High performance liquid chromatographic method (HPLC) for determination of sulfadiazine in swine meat was developed. Sulfadiazine was extracted from meat by homogenizing with acetonitrile. The homogenate was centrifuged and supermatant was evaporated. The extract was dissolved in 85% methanol and applied to an alumina coloumn. Sulfadiazine was eluted with acetonitrile-acetic acid water (22:1:78) and the eluate was applied to an HPLC coloumn packed with Nucleosil 5C18. The average recoveries of sulfadiazine added 1 ppm and 10 ppm to swine meat were 80.5% and 93.2%. The detection limit sulfadiazine by this method was 2.5ng. 0aDrugs -- PORK -- HPLC -- SULPHONAMIDES -- CALIBRATION