na INLIS000000000001401 20260309090900 0010-0326000008 ta 260309 | | | 636.09 636.09 MEU m Meutia Hayati Molecular detection of extended-spectrum ?-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia Surabaya : Veterinary World, 2019 6 hlm. : ill. ?Klebsiella pneumoniae BACTERIA RESPIRATORY DISEASES CHICKEN ANTIBIOTIC Agustin Indrawati Ni Luh Putu Ika Mayasar Istiyaningsih Neneng Atikah Background and Aim: ?Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae may be transferred to other bacteria. One of the known resistance genes is extended-spectrum ?-lactamase (ESBL). This research aimed to study K. pneumoniae isolated from chicken farms in East Java, Indonesia, by observing the antibiotic resistance pattern and detect the presence of ESBL coding gene within the isolates. Materials and Methods: A total of 11 K. pneumoniae isolates were collected from 141 chicken cloacal swabs from two regencies in East Java. All isolates were identified using the polymerase chain reaction method. Antimicrobial susceptibility was determined by agar dilution method on identified isolates, which then processed for molecular characterization to detect ESBL coding gene within the K. pneumoniae isolates found. Results: The result of antibiotic sensitivity test in 11 isolates showed highest antibiotic resistance level toward ampicillin, amoxicillin, and oxytetracycline (100%, 100%, and 90.9%) and still sensitive to gentamicin. Resistance against colistin, doxycycline, ciprofloxacin, and enrofloxacin is varied by 90.9%, 54.5%, 27.3%, and 18.2%, respectively. All isolates of K. pneumoniae were classified as multidrug resistance (MDR) bacteria. Resistance gene analysis revealed the isolates harbored as blaSHV (9.1%), blaTEM (100%), and blaCTX-M (90.9%). Conclusion: All the bacterial isolates were classified as MDR bacteria and harbored two of the transmissible ESBL genes. The presence of antibiotic resistance genes in bacteria has the potential to spread its resistance properties. https://www.veterinaryworld.org/Vol.12/April-2019/14.pdf 2231-0916 08/R/2026