02706     2200313   4500001002100000005001500021035002000036007000300056008003900059082001100098084001700109100001800126245014200144260004000286300001900326650002700345650001300372650002500385650001200410650001500422700002200437700002800459700001800487700001800505520178000523856006102303022001402364990001402378INLIS00000000000140120260309090900  a0010-0326000008ta260309                |          | |    a636.09  a636.09 MEU m0 aMeutia Hayati1 aMolecular detection of extended-spectrum ?-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia  aSurabaya :bVeterinary World,c2019  a6 hlm. :bill. 4a?Klebsiella pneumoniae 4aBACTERIA 4aRESPIRATORY DISEASES 4aCHICKEN 4aANTIBIOTIC0 aAgustin Indrawati0 aNi Luh Putu Ika Mayasar0 aIstiyaningsih0 aNeneng Atikah  aBackground and Aim: ?Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This
bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae
may be transferred to other bacteria. One of the known resistance genes is extended-spectrum ?-lactamase (ESBL). This
research aimed to study K. pneumoniae isolated from chicken farms in East Java, Indonesia, by observing the antibiotic
resistance pattern and detect the presence of ESBL coding gene within the isolates.
Materials and Methods: A total of 11 K. pneumoniae isolates were collected from 141 chicken cloacal swabs from two
regencies in East Java. All isolates were identified using the polymerase chain reaction method. Antimicrobial susceptibility
was determined by agar dilution method on identified isolates, which then processed for molecular characterization to detect
ESBL coding gene within the K. pneumoniae isolates found.
Results: The result of antibiotic sensitivity test in 11 isolates showed highest antibiotic resistance level toward ampicillin,
amoxicillin, and oxytetracycline (100%, 100%, and 90.9%) and still sensitive to gentamicin. Resistance against colistin,
doxycycline, ciprofloxacin, and enrofloxacin is varied by 90.9%, 54.5%, 27.3%, and 18.2%, respectively. All isolates of
K. pneumoniae were classified as multidrug resistance (MDR) bacteria. Resistance gene analysis revealed the isolates
harbored as blaSHV (9.1%), blaTEM (100%), and blaCTX-M (90.9%).
Conclusion: All the bacterial isolates were classified as MDR bacteria and harbored two of the transmissible ESBL genes.
The presence of antibiotic resistance genes in bacteria has the potential to spread its resistance properties.  ahttps://www.veterinaryworld.org/Vol.12/April-2019/14.pdf  a2231-0916  a08/R/2026