02599     2200301   4500001002100000005001500021035002000036007000300056008003900059082001100098084001700109100001900126245016900145260006200314300002000376650001500396650001300411650001400424650001200438700002100450700002900471700002400500700002300524520169300547856002902240022001402269990001402283INLIS00000000000139820260305102429  a0010-0326000005ta260305                |          | |    a636.09  a636.09 ERN d0 aErnes Andesfha1 aDetection of Salmonella pathogenicity island and Salmonella plasmid virulence genes in Salmonella Enteritidis originated from layer and broiler farms in Java Island  bJOURNAL OF ADVANCED VETERINARY AND ANIMAL RESEARCH,c2019  a10 hlm. :bill. 4aSalmonella 4aBACTERIA 4aDetection 4aChicken0 aAgustin Indrawat0 aNi Luh Putu Ika Mayasari0 aIrma Rahayuningtyas0 aIstiyaningsih Jusa  aObjective: The incidence of salmonellosis in humans and animals is still high due to the occurrence of virulence factors in Salmonella enterica which play a role in the process of infection in
the host and the spread of disease and most of the S. enterica can infect humans and animals. The
present study was aimed to identify Salmonella Enteritidis and detect virulence genes related to
Salmonella pathogenicity islands (SPIs) and Salmonella plasmid virulence (Spv).
Materials and Methods: A total of 27 S. Enteritidis archive isolates belonging to the National
Veterinary Drug Assay Laboratory (NVDAL) were used in this study. The bacteria were collected
in 2016 and 2017 from samples of the cloaca and fecal swabs from layer and broiler farms in five
provinces of Java Island. Isolates were cultured in specific media, biochemical tests and Gram
staining. Detection of S. Enteritidis and virulence genes was done by polymerase chain reaction
(PCR) method.
Results: Identification of serovar showed 100% (27/27) isolates were positive for the sdfI gene
(304 bp). The result confirmed that all strains were S. Enteritidis. PCR based detection of virulence
genes showed that 100% of isolates had virulence genes in SPI-1 to SPI-5, namely, invA, ssaQ,
mgtC, spi4D, and pipA genes. All the isolates (27/27) were also positive to spvB gene-based PCR.
Conclusion: All the isolates of S. Enteritidis in this study carry virulence genes related to SPI-1 to
SPI-5 and plasmid virulence. The existence of virulent genes indicates that the S. Enteritidis strain
examined in this study is highly virulent and poses a potential threat of worse disease outcome in
humans and animals.  ahttp://bdvets.org/javar/  a2311-7710  a05/R/2026