Enuh Raharjo Jusa text Bogor BBPMSOH 2004 Vol. 10, p. 1-4 id 2 tables.:7 ref.: Summary (En) The virology laboratory of Veterinary Drug Assay Laboratory isolated a virus which caused respiratory clinical signs in chicken from a layer farm, in Legok, Tangerang. The clinical signs of the disease were very similar to the avian influenza. The virus isolate was then identified by RT-PCR using primer NP-1200f and NP-1529r that amplified nucleoprotein gene of all influenza virus of poultry, man, pig and horse, and using 15 pair of H1-15 primer that amplified haemagglutinin antigen (HA) of avian subtype. The result of RT-PCR showed that both of the genes of Legok isolate were amplified with NP-1200f and NP-1529r pair and H5-131f and H5-580 pair primers. The result indicated that the Legok isolate was an avian influenza subtype H5N1. PCR -- Electrophoresis -- Layer chickens -- ISOLATION -- JAVA -- Avian Influenza Virus -- Gene -- Agglutinins 0852-9612 210323 20210323091254 INLIS000000000000078 Converted from MARCXML to MODS version 3.5 using MARC21slim2MODS3-5.xsl (Revision 1.106 2014/12/19)