01489     2200229   4500001002100000005001500021008004100036020001400077035001900091041000700110100002200117700001600139700001100155700003200166700002100198245009800219250002000317260002500337300003500362500074800397650011401145INLIS00000000000007820210323091254210323|||||||||   |   |||   |||| ||   |   a0852-9612  0010-0321000078  aid0 aEnuh Raharjo Jusa0 aPudjiatmoko0 aEmilia0 aBahruddin Syahroni Hasibuan0 aSri Murni Astuti00aIdentifikasi Subtipe Virus Avian Influenza Isolat Legok Menggunakan Polymerase Chain Reaction  aVol. 10, p. 1-4  aBogorbBBPMSOHc2004  a2 tables.:7 ref.: Summary (En)  aThe virology laboratory of Veterinary Drug Assay Laboratory isolated a virus which caused respiratory clinical signs in chicken from a layer farm, in Legok, Tangerang. The clinical signs of the disease were very similar to the avian influenza. The virus isolate was then identified by RT-PCR using primer NP-1200f and NP-1529r that amplified nucleoprotein gene of all influenza virus of poultry, man, pig and horse, and using 15 pair of H1-15 primer that amplified haemagglutinin antigen (HA) of avian subtype. The result of RT-PCR showed that both of the genes of Legok isolate were amplified with NP-1200f and NP-1529r pair and H5-131f and H5-580 pair primers. The result indicated that the Legok isolate was an avian influenza subtype H5N1. 0aPCR -- Electrophoresis -- Layer chickens -- ISOLATION -- JAVA -- Avian Influenza Virus -- Gene -- Agglutinins