na INLIS000000000000081 20210323091255 210323||||||||| | ||| |||| || | 0852-9612 010-0321000081 id Pudjiatmoko Respon pembentukan antibodi pada tikus putih terhadap vaksin ompA-Recombinant Feline Chlamydiosis vol. 8, p.8-14 Bogor BBPMSOH 2001 4 tables. 11 ref.; Summary (En) The omp A-recombinant protein produced by E.coli BL21 (DE3)plysS containing pRSETA/ompA gene of feline Chlamydia psittaci B166 strain was purified using method of Xpress system protein purification (invitrogen BV, The Netherlands). To eliminate guanidine hydrochloride, the suspension of ompA-recombinant protein was dialyzed in 20 mM Tris pH 7.8, glycerol 10% and phenylmethylsulfonyl fluoride containing 0,1 M Mgcl2, CaCl2, NaCl or KCl. To prepare the vaccines, the purified proteins were emulsified with oil adjuvant. One ml of each vaccine was injected intramuscularly to 4 spesific pathogen free rats 8-week-old. The second vaccination was performed at 3 weeks post first vaccination. IgG antibody titer against feline Chlamydia psittaci was measured using microimmunofluorescene test at a week post second vaccination. Vaccination using ompA-recombinant could initiate IgG antibody in 21 of 24 vaccinated rats. IgG antibody titer or rats vaccinated with materials dialyzed in dialysis buffer containing KCl has highest titer ranged from 1:16 to 1:64, and its geometric mean titer is 1:38. The results of the present study indicate that the ompA-recombinant protein of chlamydia used for main component of vaccine my initiate antibody against feline C. psittaci in rats Cats -- Escherichia coli -- Rats -- ANTIBODIES -- IMMUNO FLUORESCENCE -- Chlamydia Psittaci -- Bacteriosis -- Synthetic Vaccine -- Protein -- Specific Pathogen Free State