na INLIS000000000000087 20210323091256 210323||||||||| | ||| |||| || | 0852-9612 010-0321000087 id Siti Mariana ROBERT HIRST Development of an Enzyme linked immunosorbent assay for detection of antibody against pasteurella multocida in chickens vol.6 p.7-10 Bogor BBPMSOH 1999 5 tables; 11 ref. Summary (En) In order to obtain a simple, cheap and sensitive method for detecting an antibody response in vaccinated and non-vaccinated chickens an Elisa technique was developed using heat stable antigen from Pasteurella multocida P-1662 as a coating antigen. The Elisa system in this study found to be sensitive however non-spesific reaction need to be avoided. Chickens with high antibody titer need to be diluted in 1/1000, while a dilution of 1/100 could be satisfactorily used for monitoring SPF chickens for laboratory purpose. Elisa -- Spesific Pathogen Free State -- Vaccines -- CHICKENS -- ANTIBODIES -- MULTOCIDA -- ANTIGEN -- PASTEURELLA MULTOCIDA