01201     2200193   4500001002100000005001500021008004100036020001400077035001900091041000700110100001700117700001700134245012400151250001700275260002500292300003500317500052600352650012900878INLIS00000000000008720210323091256210323|||||||||   |   |||   |||| ||   |   a0852-9612  0010-0321000087  aid0 aSiti Mariana0 aROBERT HIRST00aDevelopment of an Enzyme linked immunosorbent assay for detection of antibody against pasteurella multocida in chickens  avol.6 p.7-10  aBogorbBBPMSOHc1999  a5 tables; 11 ref. Summary (En)  aIn order to obtain a simple, cheap and sensitive method for detecting an antibody response in vaccinated and non-vaccinated chickens an Elisa technique was developed using heat stable antigen from Pasteurella multocida P-1662 as a coating antigen. The Elisa system in this study found to be sensitive however non-spesific reaction need to be avoided. Chickens with high antibody titer need to be diluted in 1/1000, while a dilution of 1/100 could be satisfactorily used for monitoring SPF chickens for laboratory purpose. 0aElisa -- Spesific Pathogen Free State -- Vaccines -- CHICKENS -- ANTIBODIES -- MULTOCIDA -- ANTIGEN -- PASTEURELLA MULTOCIDA